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Titlebook: RFID Security and Privacy; Yingjiu Li,Robert H. Deng,Elisa Bertino Book 2014 Springer Nature Switzerland AG 2014

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Yingjiu Li,Robert H. Deng,Elisa Bertinoated through the immunolabeling steps, reducing the possibility of dehydration-induced antigen modification. Modern technical advancements both in preparation protocols and equipment design make cryosectioning a routine and rapid approach for immunocytochemistry that may provide increased sensitivit
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Yingjiu Li,Robert H. Deng,Elisa Bertinopophase of water (.-.). This mono-layer then is absorbed to a thin carbon film on a carbon-coated grid. To enhance contrast, the nucleic acid molecule is stained either with uranyl acetate (in particular, for dark-field microscopy) or by low-angle shadowing with evaporated heavy metal, for example,
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Yingjiu Li,Robert H. Deng,Elisa Bertinocess to this information without extracting or disrupting the location of target proteins requires specialized preparation methods. Sectioning frozen blocks of chemically fixed and cryoprotected biological material is one method for obtaining immunocytochemical data. Once the cells or tissues are cu
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Yingjiu Li,Robert H. Deng,Elisa Bertinoand subsequently in osmium tetroxide (OsO.). The specimens are then dehydrated and embedded in a (heat-polymerized) epoxy resin. While this procedure may produce excellent ultrastructural preservation (. Chapter 1), the antigenicity of most tissue proteins is severely affected. Subsequent successful
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