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Titlebook: Quantum Dots; Applications in Biol Marcel P. Bruchez,Charles Z. Hotz Book 2007 Humana Press 2007 FISH.Fluoreszenz in situ-Hybridisierung.Ge

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Optical Monitoring of Single Cells Using Quantum Dotsging capability. The multicolor capabilities of QDs allows the simultaneous monitoring of complex cellular populations, allowing more information-rich experiments to be performed. This chapter discusses the ways in which one can label cells with QDs, and a number of ways to characterize the impact that the labeling has on the cellular populations.
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Application of Quantum Dots to Multicolor Flow Cytometry of QDs (as relevant to flow cytometric applications), the advantages derived from these properties, and the procedure for conjugating antibodies to QDs. Finally, we discuss strategies for choosing the combinations of QDs and antibodies best suited for multicolor experiments.
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Marcel P. Bruchez,Charles Z. HotzIncludes supplementary material:
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Thomas J. Deerinck,Ben N. G. Giepmans,Benjamin L. Smarr,Maryann E. Martone,Mark H. Ellisman
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Single Quantum Dot Tracking of Membrane Receptorsigate the dynamics of individual GABA. receptors in the axonal growth cone of spinal neurons in culture. Single QD tracking is nevertheless a general method, suitable to study many transmembrane proteins.
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