Titlebook: Quantum Dots; Applications in Biol Marcel P. Bruchez,Charles Z. Hotz Book 2007 Humana Press 2007 FISH.Fluoreszenz in situ-Hybridisierung.Ge

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Single Quantum Dot Tracking of Membrane Receptorsegulation, or signal transduction. The advent of semi-conductor quantum dots (QDs) has opened new perspectives for the study of membrane properties because these new nanomaterials enable measurements at the single molecule level with high signal-to-noise ratio. Probes used until now indeed encounter

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Peptide-Mediated Intracellular Delivery of Quantum Dots very photostable, satisfying even imaging applications that require single molecule detection at high repetition rates over long periods of time (minutes to hours). There are by now numerous methods for conferring biospecificity and function including cell membrane permeability to QDs. A particular

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Multiple Cell Lines Using Quantum Dotsiability on a single-cell basis, together with the evaluation of cell subpopulations within wells. A high-content screening multiplexed assay format allows additional information to be gained from a single assay. One such example is the ability to determine the effects of new chemical entities on di

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Measuring Cell Motility Using Quantum Dot Probescell motility is an excellent indicator of metastatic potential. We have developed an efficient and sensitive two-dimensional cell motility assay to image the phagokinetic uptake of colloidal CdSe/ZnS semiconductor nanocrystals (quantum dots [QDs])..As cells move across a thin, homogeneous layer of

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Quantum Dots for In Vivo Molecular and Cellular Imagingliving animals. The structural design involves encapsulating luminescent QDs with an ABC triblock copolymer, and linking this polymer to tumor-targeting ligands, such as antibodies and drug-delivery functionalities. In vivo targeting studies of human prostate cancer growing in nude mouse show that t

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Sentinel Lymph Node Mapping With Type-II Quantum Dotsd, and analyzed for the presence or absence of malignant cells. Fluorescent semiconductor nanocrystals (quantum dots [QDs]) of the appropriate size, charge, and emission wavelength permit this surgery to be performed rapidly, with high sensitivity and under complete image guidance. We describe the m

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Macrophage-Mediated Colocalization of Quantum Dots in Experimental Gliomam dots (QDs). QDs are optical semiconductor nanocrystals that exhibit stable, bright fluorescence over narrow, size-tunable emission bands. The size-tunable optical properties of QDs allow multiplexing with multiple emission wavelengths from a single excitation source. QDs may be linked to antibodie

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Application of Quantum Dots to Multicolor Flow Cytometry of QDs (as relevant to flow cytometric applications), the advantages derived from these properties, and the procedure for conjugating antibodies to QDs. Finally, we discuss strategies for choosing the combinations of QDs and antibodies best suited for multicolor experiments.

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Quantum Dots for Multicolor Tumor Pathology and Multispectral Imagingem ensures optimum specimen illumination and efficient collection of reflected or emitted light and uses an innovative way of separating fluorescent emissions. Our results suggest that very weak immunoreactions seen by traditional immunohistochemical techniques can be greatly intensified, a useful feature for pathology diagnostics.