Titlebook: Quantitative PCR Protocols; Bernd Kochanowski,Udo Reischl Book 1999 Humana Press 1999

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Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standarding the amount of products during the PCR at several cycles (kinetic quantitative PCR) (.,.). In this chapter, we define these two quantitative PCR methods, give their main characteristics, and compare their advantages and drawbacks. We then give a few examples of applications of the kinetic PCR met

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Quantifying Amplicons with ELISAatility. The assay for quantifying the amount of polymerase chain reaction (PCR) products is a crucial step in any quantitative PCR method. It should be sensitive and specific, able to display a wide dynamic range, nonradioactive, easy to do, and inexpensive. The results of the assay should also be

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Amplified Assay for Specific Dual-Labeled DNA Using the Coagulation Cascade (EDNA-ELCA)popular method for identification of specific products of polymerase chain reaction (PCR) (.,.). These labeled molecules are prepared either by PCR synthesis in the presence of labeled deoxyuridine triphosphate (.,.) or by hybridization of labeled probes to the unlabeled PCR product (.,.). Detection

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