| 书目名称 | Purification of Peptides in High-Complexity Arrays |
| 副标题 | A New Method for the |
| 编辑 | Christopher Schirwitz |
| 视频video | |
| 概述 | Nominated as an outstanding Ph.D. thesis by the Institute of Applied Physical Chemistry, University of Heidelberg, and the German Cancer Research Center (DKFZ) Heidelberg, Germany.Outlines an improvem |
| 丛书名称 | Springer Theses |
| 图书封面 |  |
| 描述 | Christopher Schirwitz‘s thesis focuses on improving the quality of in situ synthesized high-complexity peptide micro arrays. Micro arrays containing proteins or small protein fragments in the form of peptides have become of great interest in proteomic research. .With the help of these microarrays a large number of potential target molecules can be screened for interaction with a probe in a short timeframe. However, protein and peptide .micro arrays are still lagging behind oligonucleotide arrays in terms of density, quality and manufacturing costs. A new approach developed at the German Cancer Research .Center (DKFZ) has improved the synthesis of high-density peptide arrays. The current technology is capable of producing arrays with up to 40,000 different peptides per square cm by means of micro particle-based solid phase peptide synthesis. However, in situ synthesis approaches bear a conceptual disadvantage: The quality of the peptides is dependent on the efficiency of the synthesis so that peptide fragments are present in the resulting array among the desired full-length peptides. In peptide-protein interaction studies such peptide fragments. The central achievement of this thesi |
| 出版日期 | Book 2013 |
| 关键词 | Cleavable Linkers; Coupling Peptides; Ellipsometry; Immunostaining; Peptide Arrays; Peptide Synthesis; Pep |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-3-319-00807-3 |
| isbn_softcover | 978-3-319-34559-8 |
| isbn_ebook | 978-3-319-00807-3Series ISSN 2190-5053 Series E-ISSN 2190-5061 |
| issn_series | 2190-5053 |
| copyright | Springer International Publishing Switzerland 2013 |
发表于 2025-3-21 18:41:48
