| 书目名称 | Proximity Labeling | | 副标题 | Methods and Protocol | | 编辑 | Murat Sunbul,Andres Jäschke | | 视频video | | | 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Proximity Labeling: Methods and Protocols. serves as an ideal guide for researc | | 出版日期 | Book 2019 | | 关键词 | Peroxidase-based labeling; BioID; Proximity ligation assay; Biophysical properties; Subcellular architec | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-9537-0 | | isbn_softcover | 978-1-4939-9539-4 | | isbn_ebook | 978-1-4939-9537-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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发表于 2025-3-21 19:47:36
