| 书目名称 | Protein‘Ligand Interactions | | 副标题 | Methods and Applicat | | 编辑 | G. Ulrich Nienhaus | | 视频video | | | 概述 | Includes supplementary material: | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. | | 出版日期 | Book 2005 | | 关键词 | biology; biosensors; microscopy; molecular biology; protein; proteins; receptor | | 版次 | 1 | | doi | https://doi.org/10.1385/1592599125 | | isbn_softcover | 978-1-61737-525-5 | | isbn_ebook | 978-1-59259-912-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2005 |
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发表于 2025-3-21 20:07:03
