书目名称 | Protein Downstream Processing | 副标题 | Design, Development, | 编辑 | Nikolaos E. Labrou | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench. The chapters in this book are divided into five parts: Part One discusses an overview of screening and design of purification strategies and covers initial aspects on high-throughput screening, methods development, and media selection; Parts Two and Three explore low- and high-resolution methods, with emphasis on affinity chromatography; Part Four describes analytical techniques of purified proteins; and Part Five presents selected examples and case studies to discuss the aforementioned. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and comprehensive, .Protein Downstream Processing: Design, Development, and Application of High and Low-Resoluti | 出版日期 | Book 2021Latest edition | 关键词 | Process Chromatography; Membrane Chromatography; ion exchange; dye-ligands; microscale chromatography pi | 版次 | 2 | doi | https://doi.org/10.1007/978-1-0716-0775-6 | isbn_softcover | 978-1-0716-0777-0 | isbn_ebook | 978-1-0716-0775-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2021 |
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