| 书目名称 | Pluripotent Stem Cells |
| 副标题 | Methods and Protocol |
| 编辑 | Uma Lakshmipathy,Mohan C Vemuri |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | .Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific or disease-specific cells for downstream applications. .Pluripotent Stem Cells: Methods and Protocols. highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods , and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering. Written i |
| 出版日期 | Book 2013 |
| 关键词 | cell replacement; cellular models; drug discovery; human embryonic stem cells; human pluripotent stem ce |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-62703-348-0 |
| isbn_softcover | 978-1-4939-6285-3 |
| isbn_ebook | 978-1-62703-348-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media New York 2013 |
发表于 2025-3-21 17:08:43
