书目名称 | Plant Virology Protocols | 副标题 | From Virus Isolation | 编辑 | Gary D. Foster,Sally C. Taylor | 视频video | | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total. | 出版日期 | Book 1998 | 版次 | 1 | doi | https://doi.org/10.1385/0896033856 | isbn_softcover | 978-1-4899-4253-1 | isbn_ebook | 978-1-59259-566-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media New York 1998 |
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