书目名称 | Plant Transcription Factors |
副标题 | Methods and Protocol |
编辑 | Ling Yuan,Sharyn E. Perry |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. .Plant Transcription Factors: Methods and Protocols. offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful .Methods in Molecular Biology™. series format, chapters con |
出版日期 | Book 2011 |
关键词 | DNA shuffling; arabidopsis protoplasts; chimeric repressors; direct target genes; gene discovery; protein |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-61779-154-3 |
isbn_softcover | 978-1-4939-5844-3 |
isbn_ebook | 978-1-61779-154-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC 2011 |