书目名称 | Plant Synthetic Promoters |
副标题 | Methods and Protocol |
编辑 | Reinhard Hehl |
视频video | |
概述 | Includes cutting-edge methods and protocols for the study of plant synthetic promoters.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | This book assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of .cis.-regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented. The experimental verification of the proposed expression profile conferred by the .cis.-regulatory elements requires the assembly of synthetic promoters. Several chapters are dedicated to the assembly of synthetic promoters, also including specific software tools to facilitate promoter design. Transient and transgenic reporter gene technology is a prominent approach to test the spatial and temporal expression driven by synthetic promoters, and several chapters address this approach. Written for the highly successful .Methods in Molecular Biology. series, chapters include the kind of detail and expert implementation advice to ensure successful results in the lab. .Practical and cutting-edge, .Plant Synthetic Promoters: Methods and Protocols .covers all steps required from the identification of .cis.-regulatory elements, over synthetic promoter design, to the |
出版日期 | Book 2016 |
关键词 | Gene expression regulation; Bioinformatics; cis-regulatory elements; Reporter genes; Transcription facto |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-6396-6 |
isbn_softcover | 978-1-4939-8186-1 |
isbn_ebook | 978-1-4939-6396-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2016 |