| 书目名称 | Pichia Protocols | | 编辑 | James M. Cregg | | 视频video | | | 概述 | All protocols are tested and readily and reproducible.Updates and expands upon the first volume.Presents the basics of Pichia pastoris and describes available vectors and host strains for expression | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .Pichia Protocols focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI)...This volume fully updates and expands upon the first edition of Pichia Protocols, and focuses primarily on information that has come to light since its original publication. Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.. | | 出版日期 | Book 2007Latest edition | | 关键词 | Expression; Fermentation; electron microscopy; genes; genetics; microscopy; Biological Microscopy | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-59745-456-8 | | isbn_softcover | 978-1-61737-563-7 | | isbn_ebook | 978-1-59745-456-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2007 |
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发表于 2025-3-21 16:28:09
