| 书目名称 | Phytochromes | | 副标题 | Methods and Protocol | | 编辑 | Andreas Hiltbrunner | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume aims at providing readers with protocols to enhance their research for the next decade of phytochrome research. The chapters in this book cover topics such as tests for light-dependent interaction of phytochrome binding proteins using yeast two hybrid assays, in vitro pull down, and co-immunoprecipitation from stable transgenic lines; investigations into phosphorylation, SUMOylation, and ubiquitination of phytochromes and other proteins; a look at how in vivo spectroscopy can be used to measure Pfr levels of phytochrome .in planta.; identifying hormonal action and co-action in light-regulated processes; and ways to use the CRISPR/Cas9 system to generate .Physcomitrella. mutant lines. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Phytochromes: Methods and Protocols. serves as a guideline to researchers who are new to the field, and a stepping stone for experienced researchers looking to ex | | 出版日期 | Book 2019 | | 关键词 | Co-IP assays; Y2H assays; ubiquitination; chromophore mutants; ChIP | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-9612-4 | | isbn_softcover | 978-1-4939-9614-8 | | isbn_ebook | 978-1-4939-9612-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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发表于 2025-3-21 18:15:36
