| 书目名称 | Phosphoinositides | | 副标题 | Methods and Protocol | | 编辑 | Roberto J. Botelho | | 视频video | | | 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | This detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell migration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful .Methods in Molecular Biology. format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Phosphoinositides: Methods and Protocols. provides detailed methodology for both specialist and novice res | | 出版日期 | Book 2021 | | 关键词 | Eukaryotic phospholipids; PtdInsPs; Enzymatic assays; Lipid bilayers; Genetically-encoded probes | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-1142-5 | | isbn_softcover | 978-1-0716-1144-9 | | isbn_ebook | 978-1-0716-1142-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2021 |
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发表于 2025-3-21 17:11:28
