| 书目名称 | PCR Cloning Protocols | | 副标题 | From Molecular Cloni | | 编辑 | Bruce A. White | | 视频video | | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. This volume focuses on such preparative PCR protocols, which can be used in the cloning and modification of DNA. PCR Cloning Protocols: From Molecular Cloning to Genetic Engineer ing is divided into seven parts, each containing a collection of chapters address ing a general approach or goal. Part I presents basic PCR protocols, emphasizing optimizing conditions for (he amplification of DNA fragments of several kilobases in length. Part 11 offers several procedures for cloning PCR prod ucts, depending on whether a specific restriction site can be used in the clon ing vector, the PCR product is to be gel purified before cloning, or the fragmeni needs to be inserted in one or both orientations. Part III includes several pro tocols involved in the mutagenesis of DNA, either site-directed or not, as well as several approaches to recombinant PCR, either for mutagenesis or building a custom | | 出版日期 | Book 19971st edition | | 版次 | 1 | | doi | https://doi.org/10.1385/0896034836 | | isbn_ebook | 978-1-59259-553-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 1997 |
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发表于 2025-3-21 18:03:33
