| 书目名称 | PCR Cloning Protocols |
| 编辑 | Bing-Yuan Chen,Harry W. Janes |
| 视频video | http://file.papertrans.cn/741/740060/740060.mp4 |
| 概述 | Includes supplementary material: |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | PCR Cloning Protocols, Second Edition, updates and expands Bruce White‘s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment. |
| 出版日期 | Book 2002Latest edition |
| 关键词 | Bur; DNA; Mutation; PCR; genes; hybridization; recombination; transcription |
| 版次 | 2 |
| doi | https://doi.org/10.1385/1592591779 |
| isbn_softcover | 978-1-61737-281-0 |
| isbn_ebook | 978-1-59259-177-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media New York 2002 |
|Archiver|手机版|小黑屋|
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