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Titlebook: Oxidants and Antioxidants; Ultrastructure and M Donald Armstrong Book 2002 Humana Press 2002

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书目名称Oxidants and Antioxidants
副标题Ultrastructure and M
编辑Donald Armstrong
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Oxidants and Antioxidants; Ultrastructure and M Donald Armstrong Book 2002 Humana Press 2002
描述In our first protocols book, Free Radical and Antioxidant Protocols (1), r- erence to in vivo, ex vivo, or in situ techniques were few compared to classical biochemical assays and only 6 of the 40 chapters were concerned with these applications. In our second book, Oxidative Stress Biomarkers and Antioxidant Protocols (2), which is being published concurrently with this third volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, the number of such chapters has increased. The literature dealing with histoche- cal/cytochemical and immunohistochemical techniques and staining to identify cellular/subcellular sites of oxidative stress has expanded rapidly, as has the molecular biology methodology used to analyze free radical and antioxidant (AOX) reactions, as well as the monitoring of living tissue. A two-way search was performed for each technique listed in Table 1, coupled with “oxidative stress” using the PUBMED search engine from the National Library of Medicine at NIH. Most of the techniques involved in m- suring oxidative stress employ molecular biology or ultrastructural approaches. Of these techniques, histology, polymerase chain reaction, and Weste
出版日期Book 2002
版次1
doihttps://doi.org/10.1385/1592592740
isbn_softcover978-1-61737-223-0
isbn_ebook978-1-59259-274-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2002
The information of publication is updating

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Measurement of Blue Fluorescence as a Protein Marker for Oxidized Membranesed at around 520 nm is due to flavin-containing components. Blue fluorescence that fluoresced at around 450 nm is believed to be derived from the reaction of proteins with lipid peroxidation products in living cells . (.).
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Tumor Necrosis Factor-α Quantification and Expression by In Situ Hybridization hybridization are invaluable. These methods allow for the successful identification of the cell types involved in the regulation and production of this cytokine. We describe modification of both methodologies that allows for detection of TNF-α protein as well as the messenger RNA (.–.) for this protein in rat brain tissue preparations.
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DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs)ividual proteins or protein complexes in DNA-binding activities can be identified. There is no single protocol that works equally for all proteins. We describe here a protocol designed to investigate DNA-binding properties of hypoxia-inducible factors (HIFs).
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