Titlebook: Osteoporosis and Osteoarthritis; Andre J. van Wijnen,Marina S. Ganshina Book 2021Latest edition Springer Science+Business Media, LLC, part

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Methods in Molecular Biologyhttp://image.papertrans.cn/o/image/704933.jpg

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https://doi.org/10.1007/978-1-0716-0989-7Skeletal degeneration; Orthopedic research; Musculoskeletal regenerative medicine; Cartilage repair; Ske

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Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cellss have the ability to be expanded and differentiated into the chondrogenic lineage in vitro. Here we describe a standardized method to expand and chondrogenically differentiate human BM-MSCs, highlighting how to overcome technical challenges and indicating the most common readout parameters to evaluate the chondrogenic differentiation capacity.

Ige326

Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Discs fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.

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Pain and Activity Measurementsvaluate pain and physical activity in mice that can be applied to preclinical orthopedic models. These methods include the von Frey filament assay that measures mechanical allodynia, open-field activity assays for evaluation of ambulation, and incapacitance measurements to determine static weight bearing.

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Osteoporosis and Osteoarthritis978-1-0716-0989-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

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