Titlebook: Oral Biology; Molecular Techniques Gregory J. Seymour,Mary P. Cullinan,Nicholas C.K. Book 2017Latest edition Springer Science+Business Med

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1064-3745 ation advice from the experts.Includes supplementary materia.This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular bioscience

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Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR)) using qPCR. It also includes the description of the most frequent problems encountered and how to solve them. In addition, a detailed protocol for multiplex qPCR to detect and quantify . and . is included.

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Generating Multiple Base-Resolution DNA Methylomes Using Reduced Representation Bisulfite Sequencinges. Here, we describe a protocol for sequencing multiple RRBS libraries in a single sequencing reaction to generate base-resolution methylomes. Furthermore, we provide a brief guideline for base-calling and data analysis of multiplexed RRBS libraries. These strategies will be useful to perform large-scale, genome-wide DNA methylation analysis.

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