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Titlebook: Optical Tweezers; Methods and Protocol Arne Gennerich Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclus

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Temperature Quantification and Temperature Control in Optical Tweezersare provided on methods to perform a temperature-independent trap calibration procedure. Finally, an effective temperature-control system is presented, and we discuss the operation of this system as well as the methods to measure the temperature at the optically trapped particle.
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Probing the Interaction Between Chromatin and Chromatin-Associated Complexes with Optical Tweezersdividual nucleosome arrays bound with chromatin-associated complexes, such as the Polycomb repressive complex 2 (PRC2). This platform comprehensively characterizes the diverse binding modes of PRC2 on chromatin, measures their mechanical strengths, and is broadly applicable to the studies of other epigenetic machineries.
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The Invention of the Lasered, and the race to build a laser eventually won by a scientist in California in 1960. It tracks the arc from Charles Townes’ success in amplifying microwaves with a device he called the maser, to graduate student Gordon Gould’s realization of how shorter and more powerful light waves could also be
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Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers Systemce detection, have accommodated a dramatic increase in the versatility of optical trapping. Quadruple (Q)-trap optical tweezers are an excellent example of such an advance, by providing three-dimensional control over two constructs and thereby enabling for example DNA-DNA braiding. However, the impl
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One-Dimensional STED Microscopy in Optical Tweezerstudying the dynamics of the biomolecular processes that these molecules are involved in. Here we provide an outline of the concurrent use of optical tweezers and fluorescence microscopy for analyzing biomolecular processes. In particular, we focus on the use of super-resolution microscopy in optical
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