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Titlebook: Oncogene-Induced Senescence; Methods and Protocol Mikhail A. Nikiforov Book 2017 Springer Science+Business Media New York 2017 Senescent ce

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发表于 2025-3-21 17:17:28 | 显示全部楼层 |阅读模式
书目名称Oncogene-Induced Senescence
副标题Methods and Protocol
编辑Mikhail A. Nikiforov
视频video
概述Includes cutting-edge methods and protocols for the study of oncogene-induced senescence.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice fr
丛书名称Methods in Molecular Biology
图书封面Titlebook: Oncogene-Induced Senescence; Methods and Protocol Mikhail A. Nikiforov Book 2017 Springer Science+Business Media New York 2017 Senescent ce
描述This book offers in a single volume a unique collection of the state-of the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and .Drosophila melanogaster., as well as offering a description of high throughput approaches. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Oncogene-Induced Senescence: Methods and Protocols. represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development..
出版日期Book 2017
关键词Senescent cells; Phenotypes; Cellular processes; Oncology; Oncoproteins
版次1
doihttps://doi.org/10.1007/978-1-4939-6670-7
isbn_softcover978-1-4939-8267-7
isbn_ebook978-1-4939-6670-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

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发表于 2025-3-21 23:29:30 | 显示全部楼层
RT-qPCR Detection of Senescence-Associated Circular RNAs, circRNAs in senescence require distinct molecular approaches. In this chapter, we describe a method for the detection and measurement of circRNAs in senescent cells using specialized reverse transcription (RT) followed by real-time quantitative (q)PCR analysis.
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Detection of Senescence Markers During Mammalian Embryonic Development,in the developing embryo. This chapter describes the methods for identifying cellular senescence in the embryo, detailing protocols for the detection of SAβ-gal activity in both sections and at the whole mount level, and immunohistochemistry protocols for the detection of additional biomarkers of senescence.
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Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senertant information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.
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Book 2017eling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and .Drosophila melanogaster., as well as offering a
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