| 书目名称 | Oligonucleotide Synthesis | | 副标题 | Methods and Applicat | | 编辑 | Piet Herdewijn | | 视频video | | | 概述 | Includes supplementary material: | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective bindingof small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. | | 出版日期 | Book 2005 | | 关键词 | DNA; PCR; gene expression; genes; siRNA | | 版次 | 1 | | doi | https://doi.org/10.1385/1592598234 | | isbn_softcover | 978-1-61737-441-8 | | isbn_ebook | 978-1-59259-823-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2005 |
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发表于 2025-3-21 19:34:47
