Titlebook: Olfactory Receptors; Methods and Protocol Fabio Marques Simoes de Souza,Gabriela Antunes Book 2018 Springer Science+Business Media, LLC, pa

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Virus-Mediated Overexpression of Vomeronasal Receptors and Functional Assessment by Live-Cell Calcig and aggression. The detection of these chemosignals involves recognition of specific ligands by dedicated G protein-coupled receptors. Here, we describe recent methodological advances using a herpes virus-based amplicon delivery system to overexpress vomeronasal receptor genes in native, dissociat

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Calcium Imaging of Individual Olfactory Sensory Neurons from Intact Olfactory Turbinates,ons (OSNs) and concha structures of mouse olfactory turbinates have greatly increased technical difficulties in research and limited applicability of certain methods. We have developed a method to monitor intracellular calcium transients of individual OSNs from intact olfactory turbinates. With this

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Numerical Models and In Vitro Assays to Study Odorant Receptors,ace and the complexity of the odorant receptor space, computational methods are in line to propose rules connecting them. We hereby propose an in silico and an in vitro approach, which, when combined are extremely useful for assessing chemogenomic links. In this chapter we mostly focus on the mining

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