Titlebook: Odontogenesis; Methods and Protocol Petros Papagerakis Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 epithel

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Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentinogenesisorter in a specific cell line and all progeny. In this protocol, we will introduce how the cell lineage tracing technique can be performed in the investigation of dentinogenesis by using .; . compound mice. Moreover, we combined cell lineage tracing in conjunction with immunofluorescence—to further

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Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilhey are a special type of subpopulation of mesenchymal stem/stromal cells (MSCs) and present subtle differences from other types of MSCs. Therefore, it requires a specialized expertise to isolate, culture, and characterize these cells in vitro and in vivo. The purpose of this chapter is to share our

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Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal Cells Subpopulations with Multiparlasts, and highly differentiated dentin-forming odontoblasts. Undifferentiated mesenchymal cells include stem/stromal cell populations usually called dental pulp mesenchymal stem cells (DP-MSCs) which differ in their self-renewal properties, lineage commitment, and differentiation capabilities. Anal

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Dental Pulp Stem Cells: Isolation, Characterization, Expansion, and Odontoblast Differentiation for or regeneration of damaged or lost tissues. Incorporation of a sufficient number of cells which do not elicit the immunoreaction in the body is a pivotal element for successful tissue formation using this method. Stem cells exhibiting strong capacity to self-renew and differentiate into different ce

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In Vitro Analysis of Intramolecular Signaling Events in PDLSCs Using Confocal and TIRF Microscopy stem cells (PDLSCs). Confocal laser scanning microscopy (CLSM) and total internal reflection fluorescence microscopy (TIRFM) are two well-studied microscopy techniques that allow an increase in the resolution and contrast of the micrographs and the capability to pinpoint events at the plasma membra

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