Titlebook: Nucleic Acid and Peptide Aptamers; Methods and Protocol Günter Mayer Book 2009 Humana Press 2009 Ligands.Protein modification.RNA.Target mo

北极人

Isolating Aptamers Using Capillary Electrophoresis–SELEX (CE–SELEX)resis (CE) as an enrichment step greatly improves the efficiency of the process. CE–SELEX is capable of isolating high-affinity aptamers in as little as 2–4 rounds of selection, shortening the process time from several weeks to as little as a few days.

NEX

Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interactionia systematic evolution of ligands by exponential enrichment (SELEX) (Schürer, et al. (2001) .. ., 47948). Here, an FCS titration experiment is described in detail, where the binding properties of tetramethylrhodamine (TMR) labelled Moenomycin A to its corresponding RNA aptamer were determined (Schürer, et al. (2001) .. ., 47948).

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Hypomania

Aptamers and Biosensorses of about 260 and 267 nM, respectively, were comparable, while the amount of bound analyte varied by a factor of 2, depending on the accessibility of the immobilized aptamer. Differences in the specificity were shown by use of the similar protein elastase.

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谷物

Nucleic Acid Pool Preparation and Characterization complexity of a nucleic acid pool can sometimes task even an experienced researcher. The following protocol takes the reader through the steps necessary for the preparation of a pool of known complexity.

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博爱家

Günter MayerProvides an easily accessible reference volume for in vitro selection procedures written by leading researchers in the field.Covers a wide range of in vitro selection protocols targeting different mol