书目名称 | Nuclear Reprogramming | 副标题 | Methods and Protocol | 编辑 | Nathalie Beaujean,Hélène Jammes,Alice Jouneau | 视频video | | 概述 | Includes fully updated, cutting-edge methods and protocols for nuclear reprogramming.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from t | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Nuclear Reprogramming: Methods and Protocols, Second Edition. includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and up-to-date, .Nuclear Reprogramming: Methods and Protocols, Second Edition. will be of interest not only to cloners but also to researchers concerned with studying the development of embryos. | 出版日期 | Book 2015Latest edition | 关键词 | Epigenetics; Nuclear transfer; Reconstructed embryos; SCNT cloning; Somatic cell genome; Somatic cell nuc | 版次 | 2 | doi | https://doi.org/10.1007/978-1-4939-1594-1 | isbn_softcover | 978-1-4939-5441-4 | isbn_ebook | 978-1-4939-1594-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media New York 2015 |
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