| 书目名称 | Nuclear Reprogramming | | 副标题 | Methods and Protocol | | 编辑 | Steve Pells | | 视频video | | | 概述 | Includes supplementary material: | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus. | | 出版日期 | Book 2006 | | 关键词 | Activation; DNA; Ethylen; Monoclonal Antibodies; PCR; Telomere; Vivo; transcription | | 版次 | 1 | | doi | https://doi.org/10.1385/1597450057 | | isbn_softcover | 978-1-61737-530-9 | | isbn_ebook | 978-1-59745-005-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2006 |
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发表于 2025-3-21 18:15:08
