Titlebook: Nuclear Bodies and Noncoding RNAs; Methods and Protocol Shinichi Nakagawa,Tetsuro Hirose Book 2015 Springer Science+Business Media New York

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书目名称Nuclear Bodies and Noncoding RNAs影响因子(影响力)




书目名称Nuclear Bodies and Noncoding RNAs影响因子(影响力)学科排名




书目名称Nuclear Bodies and Noncoding RNAs网络公开度




书目名称Nuclear Bodies and Noncoding RNAs网络公开度学科排名




书目名称Nuclear Bodies and Noncoding RNAs被引频次




书目名称Nuclear Bodies and Noncoding RNAs被引频次学科排名




书目名称Nuclear Bodies and Noncoding RNAs年度引用




书目名称Nuclear Bodies and Noncoding RNAs年度引用学科排名




书目名称Nuclear Bodies and Noncoding RNAs读者反馈




书目名称Nuclear Bodies and Noncoding RNAs读者反馈学科排名

Euthyroid

Book 2015emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that reveal how certain long non protein-coding RNAs accumulate in specific nuclear bodies and regulate the function of the bodies by serving as architectural components or controlling the dynamics of associ

Rankle

Ethics

muscle-fibers

micturition

Genome-Wide Co-Localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDally visualized by immunostaining for specific marker proteins. Although each type of nuclear body contains a distinct set of proteins, the protein components of most types of nuclear bodies remain to be identified. This chapter introduces a new approach to identify the protein components of specific types of nuclear bodies.

CONE

Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of DNA and proteins cross-linked to the RNA of interest. Here, we describe the procedure for CHART and list strategies to optimize nuclear extract preparation, capture oligonucleotide design, and isolation of nucleic acids and proteins enriched through CHART.

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Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Maspecific marker proteins. Here we describe in detail the method to identify new nuclear body protein sub-complexes through SILAC (stable isotope labeling by amino acids in culture)-based affinity purification followed by quantitative mass spectrometry.

Endoscope

Super-Resolution Imaging of Nuclear Bodies by STED Microscopyresolution of light microscopy to beyond 100 nm. Here, we describe a super-resolution technique, gated STED, for the analysis of the structure of nuclear bodies, with emphasis on the sample preparation and other technical tips that are important to obtain high-quality super-resolution images.

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