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Titlebook: Notch Signaling Research; Methods and Protocol Dongyu Jia Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licens

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发表于 2025-3-21 18:01:38 | 显示全部楼层 |阅读模式
书目名称Notch Signaling Research
副标题Methods and Protocol
编辑Dongyu Jia
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Notch Signaling Research; Methods and Protocol Dongyu Jia Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licens
描述This detailed volume explores the large research area of Notch signaling studies in a variety of model organisms. The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in development and diseases, while acknowledging the importance of combining classic and novel molecular tools and methods, bioinformatics workflows and applications, and different model organisms to fully understand the subject area. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and cutting-edge, .Notch Signaling Research: Methods and Protocols. is an ideal guide for researchers in the field, with techniques that could potentially be applied to study the wider world of other signaling pathw
出版日期Book 2022
关键词Signaling pathways; Dysregulation; Notch signaling transduction; Notch disease models; Notch regulators;
版次1
doihttps://doi.org/10.1007/978-1-0716-2201-8
isbn_softcover978-1-0716-2203-2
isbn_ebook978-1-0716-2201-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Book 2022rotocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in devel
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Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways interference-mediated knockdowns of the Notch component to be tested, reporters for two Notch-activated pericardial genes in ., immunohistochemistry, and fluorescence microscopy, we describe a method to determine the type of signaling mechanism—instructive, permissive, or both—to which a particular Notch pathway component contributes.
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An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg ogenesis is a widely used model in developmental biology for analyzing the Notch pathway. However, determining the stage of oogenesis is difficult and varies depending on individual analyzing it. Here, we provide a MATLAB tool to automatically identify the stage of a . egg chamber and reveal the Notch expression pattern.
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Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivatioprecipitation with mitotic cells, and measure the first run of RNA synthesis upon mitotic exit. These methods serve as basis to understand the roles of mitotic bookmarking by RBPJ in propagating Notch signals through cell division.
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