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Titlebook: Notch Signaling; Methods and Protocol Hugo J. Bellen,Shinya Yamamoto Book 2014 Springer Science+Business Media New York 2014 Notch activity

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发表于 2025-3-21 19:20:47 | 显示全部楼层 |阅读模式
书目名称Notch Signaling
副标题Methods and Protocol
编辑Hugo J. Bellen,Shinya Yamamoto
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Notch Signaling; Methods and Protocol Hugo J. Bellen,Shinya Yamamoto Book 2014 Springer Science+Business Media New York 2014 Notch activity
描述.Notch Signaling: Methods and Protocols. focuses on molecular, biochemical and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage and transcriptional targets. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical,. Notch Signaling: Methods and Protocols. is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor. .
出版日期Book 2014
关键词Notch activity in cells; Notch interactome; Notch signaling; genetic methods; image Notch signaling; post
版次1
doihttps://doi.org/10.1007/978-1-4939-1139-4
isbn_softcover978-1-4939-5510-7
isbn_ebook978-1-4939-1139-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
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978-1-4939-5510-7Springer Science+Business Media New York 2014
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Notch Signaling978-1-4939-1139-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
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https://doi.org/10.1007/978-1-4939-1139-4Notch activity in cells; Notch interactome; Notch signaling; genetic methods; image Notch signaling; post
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,Assay to Probe Proteolytic Processing of Notch by γ-Secretase,s become important. Here we describe a cell-based method to analyze the cleavage of Notch at the S3 site by γ-secretase. HEK cells are transfected with an N-terminal truncated and myc-labeled mNotchΔE construct which can be easily and quantitatively detected by western blotting.
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editor. The problem of random walk as it is originally stated by Pearson (Nature 72:294–294, 1905a) applied to Donsker’s (Mem Am Math Soc 6:1–10, 1951) invariance principle as a functional extension of central limit theorem. The Kalman filter approach together with its extended dynamic version capt
发表于 2025-3-23 02:33:05 | 显示全部楼层
Shinya Yamamoto,Karen L. Schulze,Hugo J. Belleneasure, which was the result of a compromise between Congress and the Truman administration, threatened the . of multilateral cooperation between the United States and its Western European allies in East-West trade policy. Forcing nations receiving economic and military assistance from Washington to
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