Titlebook: Nitrogen Fixation; Methods and Protocol Markus W. Ribbe Book 2011 Springer Science+Business Media, LLC 2011 Biogenesis.Biosynthetic mechani

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书目名称Nitrogen Fixation影响因子(影响力)




书目名称Nitrogen Fixation影响因子(影响力)学科排名




书目名称Nitrogen Fixation网络公开度




书目名称Nitrogen Fixation网络公开度学科排名




书目名称Nitrogen Fixation被引频次




书目名称Nitrogen Fixation被引频次学科排名




书目名称Nitrogen Fixation年度引用




书目名称Nitrogen Fixation年度引用学科排名




书目名称Nitrogen Fixation读者反馈




书目名称Nitrogen Fixation读者反馈学科排名

树胶

Mechanism of Mo-Dependent Nitrogenaseobal biogeochemical nitrogen cycle, this enzyme has been the focus of intensive research for over 60 years. This chapter provides an overview of the features of nitrogenase as a background to the subsequent chapters of this volume that detail the many methods that have been applied in an attempt to

STAT

FLORA

Genomic Analysis of Nitrogen Fixationhave enabled thorough analysis of the gene organization of nitrogen-fixing species, the identification of new genes involved in nitrogen fixation, and the identification of new diazotrophic species. This chapter reviews key characteristics of nitrogen-fixing genomes and methods to identify and analy

监禁

Enzymatic Systems with Homology to Nitrogenaseorophyllide during chlorophyll biosynthesis. Formation of bacteriochlorophyll additionally requires a structurally related enzyme system which is termed chlorophyllide oxidoreductase (COR). During DPOR catalysis, the homodimeric subunit ChlL. transfers electrons to the corresponding heterotetrameric

Stricture

Molecular Biology and Genetic Engineering in Nitrogen Fixationdied diazotrophs, . is the best studied for its convenience of aerobic growth, its high levels of nitrogenase expression, and its genetic tractability. This chapter includes protocols and strategies in the molecular biology and genetic engineering of . that have been used as valuable tools for advan

震惊

Purification of Nitrogenase Proteinsree metal centers that are critical for nitrogenase activity. The inherent complexity of this enzyme system, which is further compounded by the sensitivity of the metal clusters toward oxygen, makes the large-scale purification of fully active nitrogenase proteins a formidable task. This chapter hig

Magisterial

Assays of Nitrogenase Reaction Productse (MgATP), a suitable source of reductant, a buffer system compatible with the product-quantification protocol to be used, and the desired substrate. The assay is initiated by injection of the component protein(s) of the enzyme or MgATP and terminated by injection of either acid or a solution of Na.

STEER

Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductasegymnosperms, ferns, algae, and photosynthetic bacteria. During protochlorophyllide (Pchlide) reduction, the homodimeric subunit ChlL. of DPOR transfers electrons on the corresponding heterotetrameric catalytic subunit (ChlN/ChlB).. Although DPOR shares significant amino acid sequence homology to the

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