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Titlebook: Nitric Oxide; Methods and Protocol Helen O. McCarthy,Jonathan A. Coulter Book 2011 Springer Science+Business Media, LLC 2011 Biofilms.Detec

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发表于 2025-3-21 18:45:50 | 显示全部楼层 |阅读模式
书目名称Nitric Oxide
副标题Methods and Protocol
编辑Helen O. McCarthy,Jonathan A. Coulter
视频video
概述Addresses the challenges of accurate quantification of nitric oxide in either in vitro or in vivo models with practical, step-by-step protocols.Includes readily reproducible techniques crafted by lead
丛书名称Methods in Molecular Biology
图书封面Titlebook: Nitric Oxide; Methods and Protocol Helen O. McCarthy,Jonathan A. Coulter Book 2011 Springer Science+Business Media, LLC 2011 Biofilms.Detec
描述The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule.In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory.Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropat
出版日期Book 2011
关键词Biofilms; Detection and quantification; In vivo generation; Nanoparticles; Nitrate; Physiological, patho
版次1
doihttps://doi.org/10.1007/978-1-61737-964-2
isbn_softcover978-1-4939-5791-0
isbn_ebook978-1-61737-964-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2011
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Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/666629.jpg
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Quantum Cascade Laser Technology for the Ultrasensitive Detection of Low-Level Nitric Oxideetection limit in ppbv and sub-ppbv range. We will describe here a selection of the most effective techniques, i.e., laser absorption spectroscopy, cavity-enhanced spectroscopy, photoacoustic spectroscopy, and Faraday modulation spectroscopy. For each technique, advantages and drawbacks will be underlined.
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Ionizing Radiation-Induced DNA Strand Breaks and γ-H2AX Foci in Cells Exposed to Nitric Oxidepoints to a role for DNA damage and repair in this phenomenon. We have recently observed that nitric oxide enhances the formation of DNA single- and double-strand breaks following ionising irradiation, measured by the alkaline comet assay and immunofluorescence microscopy for γ-H2AX.
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Determination of S-Nitrosothiols in Biological Fluids by Chemiluminescencen, leukocyte adhesion, and for cellular signaling. However, because of the low levels of s-nitrosothiols and interference with other nitric oxide species, reliable assays that measure both high molecular weight and low molecular weight s-nitrosothiols in plasma and red blood cells have been difficul
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