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Titlebook: Nitric Oxide; Methods and Protocol Alexander Mengel,Christian Lindermayr Book 2018 Springer Science+Business Media, LLC, part of Springer N

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发表于 2025-3-21 16:46:39 | 显示全部楼层 |阅读模式
书目名称Nitric Oxide
副标题Methods and Protocol
编辑Alexander Mengel,Christian Lindermayr
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Nitric Oxide; Methods and Protocol Alexander Mengel,Christian Lindermayr Book 2018 Springer Science+Business Media, LLC, part of Springer N
描述.This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part one looks at NO treatments using gaseous nitric oxide and detection using a NO-sensitive electrode, electron spin resonance, and fluorescence-based NO-sensor proteins. Part two talks about various techniques used to detect and identify NO-dependent modifications, such as biotin-switch assay and quantification of s-nitrosated proteins. Part three focuses on the study of s-nitrosothiol homeostasis and denitrosation activities. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting edge and authoritative, .Nitric Oxide: Methods and Protocols. is a valuable resource for anyone interested in learning more about this evolving field..
出版日期Book 2018
关键词nitrate reductase; xanthine oxidase; polyamine oxidase; electron spin resonance; mass spectrometry
版次1
doihttps://doi.org/10.1007/978-1-4939-7695-9
isbn_softcover978-1-4939-9260-7
isbn_ebook978-1-4939-7695-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
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发表于 2025-3-21 23:41:07 | 显示全部楼层
Measurements of Intra-oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrodear [NO] measurement utilizing an L-shaped amperometric integrated NO-selective electrode. This method not only provides an elegant and convenient approach to real-time the measurement of NO in physiological environments but also mimics the loss of NO caused by rapid NO diffusion combined with its reactivity in the biological milieu.
发表于 2025-3-22 03:57:37 | 显示全部楼层
Detection of Nitric Oxide via Electronic Paramagnetic Resonance in Mollusksxclusively associated to an enzyme family known as Nitric Oxide Synthases (NOSs). The digestive glands of mollusks are a major target for oxidative disruption related to environmental stress. A simple EPR-methodology to asses both, the presence of NO and its rate of generation in tissues from different mollusk species, is reported here.
发表于 2025-3-22 07:59:04 | 显示全部楼层
Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Techniquen made possible by the development of the Biotin-Switch Technique (BST) in the early 2000s. Here, we describe the BST protocol we routinely use to check in vitro S-nitrosylation of recombinant proteins induced by NO donors.
发表于 2025-3-22 10:51:10 | 显示全部楼层
A Simple and Useful Method to Apply Exogenous NO Gas to Plant Systems: Bell Pepper Fruits as a Models. In this chapter a method to treat plant materials with NO is provided using bell pepper fruits as a model. This method is cheap, free of side effects, and easy to apply since it only requires common chemicals and tools available in any biology laboratory.
发表于 2025-3-22 13:14:13 | 显示全部楼层
Real-Time Imaging of Nitric Oxide Signals in Individual Cells Using geNOps NO. signals. Here, we describe detailed methods suitable for imaging of NO. signals in mammalian cells. This novel approach may facilitate a broad range of studies to (re)investigate the complex NO. biochemistry in living cells.
发表于 2025-3-22 19:02:39 | 显示全部楼层
Direct Measurement of S-Nitrosothiols with an Orbitrap Fusion Mass Spectrometer: S-Nitrosoglutathionrapid assay to estimate protein SNO abundance with intact protein electrospray ionization mass spectrometry. Originally designed using recombinant GSNOR, these methods are applicable to any purified protein to test for or further study nitrosatable cysteines.
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发表于 2025-3-23 04:47:06 | 显示全部楼层
1064-3745 ation advice from the experts.This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part on
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