Titlebook: Nipah Virus; Methods and Protocol Alexander N. Freiberg,Barry Rockx Book 2023 Springer Science+Business Media, LLC, part of Springer Nature

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Primary Culture of the Human Olfactory Neuroepithelium and Utilization for Henipavirus Infection In ed in the neuroepithelium of the nasal cavities and directly connect the nasal cavity with the central nervous system (CNS) via axons, which traverse the cribriform plate to synapse within the olfactory bulb. ORNs are derived from precursor cells that lie adjacent to the basal lamina of the olfactor

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In Vivo Imaging of Nipah Virus Infection in Small Animal Rodent Modelsin an individual animal over time. Recombinant henipaviruses expressing bioluminescent or fluorescent reporter proteins can be used both to monitor the spatial and temporal progression of Nipah virus (NiV) infection in vivo as well as in ex vivo tissues. Virally produced luciferases react with syste

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Nonhuman Primate Models for Nipah and Hendra Virus Countermeasure Evaluationspectively. These viruses are considered Category C pathogens by the US Centers for Disease Control. Nipah virus was recently placed on the World Health Organization Research and Development Blueprint Roadmaps for vaccine and therapeutic development. Given the infrequent and unpredictable nature of

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Nipah Virus Aerosol Challenge of Three Distinct Particle Sizes in Nonhuman Primatesrough aerosol in a laboratory-controlled environment. Inhalational studies involving Nipah virus with small (1–3 μm), intermediate (6–8 μm), and large particles (10–14 μm) were explored in African green nonhuman primates to determine if the subsequent disease course more closely recapitulated what i

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Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pan viruses that were once difficult to study, because finding suitable animal models of infection can be challenging, cost prohibitive, and often do not entirely recapitulate all parameters of the disease. Here, we describe the procedure of human immune system reconstitution (humanization) of NOD.Cg

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Ferret Models for Henipavirus Infectiones. The mechanism by which these viruses can cause disease remain largely unknown and to date there are no therapeutics or vaccines approved for use in humans. Nipah virus is listed on the World Health Organization R & D Blueprint list of epidemic threats. In order to advance the availability of eff

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