Titlebook: New Horizons in Nitrogen Fixation; Proceedings of the 9 Rafael Palacios,Jaime Mora,William E. Newton Conference proceedings 1993 Springer S

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野蛮

Gene Products and Their Roles in Nitrogen Fixationsince the Knoxville meeting than in any period following the original designation of gene product relationships by Roberts et al. (1978). Several . gene products have been isolated, the biochemical properties of some have been determined and the roles of a number of the products have been establishe

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Use of Fe Protein Altered at Specific Amino Acid Positions to Probe Its Function in Nitrogenase Cataal years of research (Mortenson et al, 1992). The ordered steps of its involvement in nitrogen fixation are as follows: (a) MgATP binds at its two mononucleotide binding sites; (b)its single oxidized 4Fe-4S center accepts an electron from an electron donor; (c) it binds to the MoFe protein (MoFeP);

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Metal Regulation of Nitrogenases and Molybdenum Transport in ,that . could grow in the absence of both Mo and V (Bortels 1930; 1936). For 50 years these results were misinterpreted as showing that nitrogenase has an absolute requirement for Mo, when iron alone is the only transition metal needed; though the efficiency of fixation is significantly higher with V

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Regulatory Role of Molecular Chaperones in Nitrogenase Biogenesisions indicating that the assembly of NifD and NifK, co-expressed in non-diazotrophic organisms, was host-dependent first suggested to us the possibility that host molecular chaperones played a role in nitrogenase biogenesis (Holland et al., 1987). Molecular chaperones are proteins that assist the co

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The Role of Respiration in Symbiotic Nitrogen Fixationectly from the quinol pool, whereas cytochrome . oxidases receive the electrons from cytochrome . that has been reduced before by ubiquinol-cytochrome . oxidoreductase (also called cytochrome .. complex; Trumpower 1990). Hence, when bacteria employ two or more terminal oxidases, the respiratory chai

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An Integrated, Interdisciplinary Approach to Understanding the Chemistry of Nitrogenasesanalytical data and results from the careful monitoring of the effects of site-specific and auxiliary gene mutagenesis, until very recently, provided the only insight into the structure and protein environment of these prosthetic groups (see Smith, Eady 1992 and Lowe 1992 and references therein). In