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Titlebook: New Aspects in Analyzing the Synaptic Organization of the Brain; Joachim H.R. Lübke,Astrid Rollenhagen Book 2024 The Editor(s) (if applica

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Agnes Korcsak-Gorzo,Charl Linssen,Jasper Albers,Stefan Dasbach,Renato Duarte,Susanne Kunkel,Abigail
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Neuromethodshttp://image.papertrans.cn/n/image/664832.jpg
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https://doi.org/10.1007/978-1-0716-4019-73D synaptic organization; electron microscopy; Neuromodulation; neurotransmitter receptor distribution;
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Joachim H.R. Lübke,Astrid RollenhagenIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
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Modern Glyoxal Fixation for the Determination of the Copy Number of Synaptic Vesicle Proteins at Indhat was purified from glyoxylic acid. The composition of the presented fixative was created to optimize acid-free glyoxal fixation for increased sensitivity of antibody labeling while preserving the fine structure at the highest possible level. The protocol describes the use of acid-free glyoxal for
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Unbiased Sampling and Volume-Sorting of Origin-Specific Terminals Using SBEM Image Stacks attainable image stacks can be used for studying the circuitry within the given nuclei and the contributions of different synaptic boutons whose origins can be identified by morphological or morphometric criteria using random sampling strategies. Here we describe an unbiased axon terminal sampling
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