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Titlebook: Neutrophil; Methods and Protocol Mark T. Quinn,Frank R. DeLeo Book 2020Latest edition Springer Science+Business Media, LLC, part of Springe

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书目名称Neutrophil
副标题Methods and Protocol
编辑Mark T. Quinn,Frank R. DeLeo
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Neutrophil; Methods and Protocol Mark T. Quinn,Frank R. DeLeo Book 2020Latest edition Springer Science+Business Media, LLC, part of Springe
描述.Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of .Neutrophils: Methods and Protocols., chapters retained from previous editions have been have been updated and include many new approaches.  In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions.  Part I contains overviews of neutrophil biology, function, and disorders.  Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity.  Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis.  Part V provides multip
出版日期Book 2020Latest edition
关键词Venous Blood; (3D) Culturing; Eosinophils; SIGNAL TRANSDUCTION; Rap GTPase; Extracellular Matrix
版次3
doihttps://doi.org/10.1007/978-1-0716-0154-9
isbn_softcover978-1-0716-0156-3
isbn_ebook978-1-0716-0154-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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Isolation of Human Neutrophils from Venous Bloodensities of the leukocytes circulating in venous blood, one can separate leukocytes from erythrocytes as well as isolate the individual leukocyte populations in high purity for use in ex vivo studies. For selected functional studies, such as transcriptional analysis or cytokine quantitation, additio
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Isolation of Neutrophils from Larval Zebrafish and Their Transplantation into Recipient Larvae for Fntrol neutrophil function. To resolve whether a gene contributes to neutrophil function in a cell-autonomous manner necessitates a way to examine gene-deficient neutrophils in an otherwise wild type background. To this end, here we describe methods to harvest fluorescent neutrophils from larval dono
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Ex Vivo Human Neutrophil Swarming Against Live Microbial Targets could not cover. The concerted actions of neutrophils are essential for the expeditious protection of healthy tissues from wounds and microbes. These processes, collectively known as swarming, are typically studied in vivo in mice. However, these studies are low throughput and their relevance to hu
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