Titlebook: Neuronal Morphogenesis; Methods and Protocol Kazuhito Toyooka Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive li

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Interrogating the Molecular Clutch in Neuronal Growth Cones: Measuring Traction Forces, F-actin Rethis process involves the attachment of the growth cone to the substrate and the cyclical engagement/disengagement of the molecular clutch at the sites of adhesive contact. In this chapter, we describe protocols for traction force microscopy, measurement of F-actin retrograde flow velocities, and the

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Measuring Retrograde Actin Flow in Neuronal Growth Cones,euronal growth cones. The direction of the actin flow is generally retrograde from the periphery toward the center of the cell. Actin flow can be harnessed for forward movement of the cell through substrate-cytoskeletal coupling; thus, a key function of actin flow is in cell locomotion. In this chap

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Morphological Analysis of Neurons and Glia Using Mosaic Analysis with Double Markers,ue-wide roles of candidate genes with single cell resolution. Given the relatively sparse labeling, depending on which of the 19 MADM chromosomes one chooses, the MADM approach represents the perfect opportunity for cell morphology analysis. Various MADM studies include reports of morphological anom

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