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Titlebook: Neuronal Cell Culture; Methods and Protocol Shohreh Amini,Martyn K. White Book 2021Latest edition Springer Science+Business Media, LLC, par

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发表于 2025-3-21 18:35:39 | 显示全部楼层 |阅读模式
书目名称Neuronal Cell Culture
副标题Methods and Protocol
编辑Shohreh Amini,Martyn K. White
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Neuronal Cell Culture; Methods and Protocol Shohreh Amini,Martyn K. White Book 2021Latest edition Springer Science+Business Media, LLC, par
描述.This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved.  Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques.  Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.  .
出版日期Book 2021Latest edition
关键词SH-SY5Y neuroblastoma cells; embryonic neural progenitor cells; cortical neurons; ; cervical ganglia; dor
版次2
doihttps://doi.org/10.1007/978-1-0716-1437-2
isbn_softcover978-1-0716-1439-6
isbn_ebook978-1-0716-1437-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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Nune Darbinian,Armine Darbinyan,Nana Merabova,Shohreh Amini
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Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications,g primary NSCs directly from fetal tissue is still the most common technique for preparation and study of neurons. Regardless of the source of tissue, similar techniques are used to maintain NSCs in culture and to differentiate NSCs toward mature neural lineages. This chapter will describe specific
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Slice Culture Modeling of CNS Viral Infection,egies. We describe techniques to (1) generate brain and spinal cord slices from rodent donors, (2) virally infect slices, (3) monitor viral replication, (4) assess virally induced injury/apoptosis, (5) characterize “CNS-specific” cytokine production, and, (6) treat slices with cytokines/pharmaceutic
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,Neurospheres and Glial Cell Cultures; from Plating to Cell Phenotyping, the homeostasis of the brain by providing the source to replace differentiated cells that have been lost or damaged by different pathological processes, such as injury, genetic conditions, or disease. The discovery of these Neural Stem Cells in an organ traditionally thought to have limited or no r
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