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Titlebook: Neurocytochemical Methods; André Calas,Daniel Eugène Book 1991 Springer-Verlag Berlin Heidelberg 1991 PET.anatomy.brain.forebrain.morpholo

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Investigating the Three-Dimensional Morphology of Fluorescence-Labelled Neurons Using Confocal Lasermensional (3D) morphology of fluorescence-labelled neurons in the central nervous system of the lamprey, a lower vertebrate. Intracellular recordings were performed in an . preparation of the lamprey CNS with microelectrodes filled with Lucifer Yellow. After intracellular dye-injection of identified
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Molecular Neuroanatomy of Synapses, Cells and Systems in the Brainl properties of cells. It provides information on the physical constraints imposed by the brain on neuronal circuits in meeting their functional demands. Thanks to tremendous technical advances, molecular neuroanatomy often paves the way for functional questions tackled by other disciplines, and it
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Regulated Expression of Transcription Factor and Neurotransmitter Genes in Neural Populations: Studinctional activity of the expressing neurons (Uhl and Nishimori, 1990). The brain expresses more different genes than any other organ in the body. Much of this complexity may relate to the expression of different sets of genes by different neuronal populations. Studies of both the nature and the mech
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The Use of Markers to Study the Regulation of Glial Cells in the PNS and CNSmarkers for the different cell types. The particular combination of antibodies chosen depends very much on the developmental stage and anatomical area of the nervous system chosen, on whether the cells are being studied . or in culture, and on the question that is being asked. In studies of glia fro
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Applications to the Study of Acetylcholine Release of the Choline Oxidase Acetylcholine Assaye, 1985), as alternatives to methods relying on radioactive labels. A chemiluminescent method to measure acetylcholine (ACh) has been developped by Israel and Lesbats (1981, 1985, 1987). Oxidation of luminol by H202 in the presence of peroxidase produces a blue light emission. Several instruments, l
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Positron Emission Tomography: Functional Neuroanatomy ,ticals in brain tissue, following the intravenous or inhalational administration of a molecule labeled with a positron emitting radionuclide. The main advantages of PET relative to other functional imaging methods are: 1) the availability of a wide array of compounds naturally labeled with .O, .C, .
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Report on the Discussion of the Third Session: Quantitative Aspectsor the assay of choline and acetylcholinesterase for the hydrolysis of acetylcholine. In this way both compounds can be assayed in the same sample. This method will also allow the determination of the ACh during release. KC1 was found to release only a small proportion of ACh because the Ca. channel
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