Titlebook: Neuroanatomical Techniques; Insect Nervous Syste N. J. Strausfeld,Thomas A. Miller Book 1980 Springer-Verlag New York Inc. 1980 Insekten.Ne

paragon

AVOID

The Bodian Protargol Technique,ed silver-on-the-slide techniques. In the earlier block impregnation procedures, such as those of Golgi, Cajal, and Bielschowsky, and their modifications, whole pieces of tissue are treated with silver solutions and only afterward sectioned and mounted. In the silver-on-the-slide methods the materia

craven

Reduced Silver Impregnations Derived from the Holmes Technique,e to give consistent results with arthropod materials, and the best results obtained were usually far inferior to those from vertebrate preparations. This chapter describes a number of modifications of Holmes’ method for vertebrate peripheral nerve (Holmes, 1947). The original method was first appli

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CROW

The Golgi Method: Its Application to the Insect Nervous System and the Phenomenon of Stochastic Imp respect to strategies of fixation, chromation, and metal impregnation. This is followed by discussions of a possible mechanism of impregnation based on direct observations of neurons as they are impregnated and on model substrates. It is argued that silver (or mercury) Chromate precipitates are not

LEVER

Electron-Microscopic Methods for Nervous Tissues,Thus examination of thin sections of biological tissue at high resolution (2.0 nm or less) with the electron microscope has been possible for a quarter of a century. Since the early fifties there have been dramatic improvements in the techniques of fixation, embedding, and sectioning, and these have

aesthetic

LEVER

OCTO

Instantaneous

Intracellular Staining with Fluorescent Dyes,of the spectrum most of the neurons present are stained to reveal the gross architecture of the brain. Speculations can then be made about the connectivity between those cells that appear to come into close association. At the other end of the spectrum individual cells are stained selectively, revea