Titlebook: Natural Products Isolation; Satyajit D. Sarker,Zahid Latif,Alexander I. Gray Book 20052nd edition Humana Press 2005 chromatography.crystal

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Methods in Biotechnologyhttp://image.papertrans.cn/n/image/661894.jpg

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https://doi.org/10.1385/1592599559chromatography; crystal; extraction; natural product

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Satyajit D. Sarker,Zahid Latif,Alexander I. GrayIncludes supplementary material:

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Natural Products Isolation978-1-59259-955-4Series ISSN 1940-6061 Series E-ISSN 1940-607X

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Isolation of Natural Products by Low-Pressure Column Chromatography, provided. Sixty-one specific examples of the use of LPLC for the isolation of various natural products, including flavones, coumarins, alkaloids, and triterpenoid saponins from various plants are given. Many of these examples incorporate a combination of adsorption and size-exclusion chromatography to obtain specific compounds.

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Hyphenated Techniques,-line detection of natural products, chemotaxonomic studies, chemical fingerprinting, quality control of herbal products, dereplication of natural products, and metabolomic studies are discussed with appropriate examples. Particular emphasis is given on the hyphenated techniques that involve liquid chromatography, as the separation tool.

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Purification by Solvent Extraction Using Partition Coefficient,ounds from crude natural product extracts. In this chapter, the generic protocols of this separation technique in the purification of natural compounds, especially phytochemicals of relatively small molecular weights, i.e., in the range of 100–1500 Da, have been outlined.

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