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Titlebook: Natural Products Isolation; Satyajit D. Sarker,Zahid Latif,Alexander I. Gray Book 20052nd edition Humana Press 2005 chromatography.crystal

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Hyphenated Techniques,provements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products. In this chapter, recent advances in the applications of various hyphenated techniques, e.g., GC-MS, LC-PDA, LC-MS, LC-FT
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Crystallization in Final Stages of Purification,as a purification step in its own right, or to produce crystals for molecular structure determination by single-crystal or powder X-ray diffraction. Both aspects are discussed, with particular emphasis on growing crystals for structure determination in natural product chemistry. The processes detail
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Dereplication and Partial Identification of Compounds, are being driven by the need to identify novel pharmaceutical and agrochemical lead compounds rapidly and effectively from complex natural matrices, while avoiding spending time or money on the re-isolation and re-identification of known natural products. As many commercial pharmaceuticals and agro
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Extraction of Plant Secondary Metabolites,tions presents specific challenges that must be addressed throughout the solvent extraction process. Successful extraction begins with careful selection and preparation of plant samples, and thorough review of the appropriate literature for indications of which protocols are suitable for a particula
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Isolation of Marine Natural Products,trial and academic groups are accessing this source using advanced technology platforms. The purpose of this chapter is to offer some practical guidance in the process of extraction and isolation of marine natural products. Special attention is given to the isolation procedures adapted to the physic
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Isolation of Microbial Natural Products,c components produced can be distributed between both the solid and liquid phases. A major challenge facing the practitioner attempting to isolate a microbial natural product, where the organic component is extracellular and has been exuded into the medium, is the separation of the desired organic c
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Purification of Water-Soluble Natural Products, researchers. A general approach to extract and fractionate water-soluble compounds from biological materials and fluids including seawater is presented in this chapter. Some important techniques and special caution needed to deal with water-soluble compounds are also included. The chapter also pres
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