Titlebook: Natural Killer (NK) Cells; Methods and Protocol Noriko Shimasaki Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive

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In Vitro Development of Mouse and Human NK Cells from Hematopoietic Progenitor Cells immunotherapy have generated great interest, given the promising results of NK cell adoptive transfer clinical trials. The remaining challenge to bring emerging NK cell immunotherapies to the clinic is to enhance the production of large numbers of functionally competent NK cells ex vivo. Here, we d

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Induction of Human Natural Killer Cells Under Defined Conditions by Seamless Transition from Maintens of NK cell-associated diseases and in the production of off-the-shelf cellular medicines. We have developed a method for the differentiation of NK cells from human PSCs under serum-free and two-dimensional condition. Our method enables the seamless transition from maintenance of PSCs to differenti

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Antibody–Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10× Genomics’ particular, the overlay of cell-surface proteome data with gene expression analysis presents a necessary advancement, particularly in the field of immunology. Here we describe a copper-free click chemistry method for the generation of antibody-oligonucleotide complexes and present the steps for its

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