Titlebook: Nanotoxicity; Methods and Protocol Qunwei Zhang Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Nanotoxicology

先锋派

cortex

Preparation and Utilization of a 3D Human Liver Microtissue Model for Nanotoxicological Assessment,ls (NM). With the ever increasing public and occupational exposure associated with accumulative production of nanomaterials, there is an urgent need to consider the possibility of harmful health consequences of engineered NM exposure. It is understood that following exposure via inhalation, ingestio

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Deriving Neural Cells from Pluripotent Stem Cells for Nanotoxicity Testing,tiation can faithfully recapitulate stages of neural development and generate neuronal progenitors, mature neurons, and glial cells. Stem cell technology will largely allow for the replacement of animal studies and reduce costs, and will provide a new paradigm for in toxic genomics, bioinformatics,

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休息

immunity

谄媚于人

Application of Gelatin Zymography in Nanotoxicity Research, biological sources. It has been used particularly to detect the two members of the matrix metalloproteinase family, MMP-2 (gelatinase A) and MMP-9 (gelatinase B), due to their potent gelatin-degrading activity. MMP-2 and MMP-9 are also able to degrade a number of extracellular matrix molecules incl

CHOIR

Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies,enomic instability, and even tumorigenesis if not repaired efficiently. In response to DNA damage, histone H2AX molecules are rapidly phosphorylated at serine 139 near the site of DNA DSBs and form γ-H2AX foci. As an early important cellular event linked to DNA damage and repair, γ-H2AX is a highly

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Analysis of Nanomaterial Toxicity by Western Blot,tive method for protein analysis that involves the solubilization and separation of proteins by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), transferring and immobilizing proteins onto a solid support, and targeted immunoprobing of a specific antigen. As a convenient and rel

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Quantification of Mitochondrial Genome Transcription in Male Reproductive Cells by Real-Time PCR,lved in important physiological processes, such as the three-carboxylic acid cycle, the oxidation of fatty acids and amino acids, and calcium ion homeostasis. The energy demands of male germ cells during mitosis and meiosis are higher than those of somatic cells, suggesting that mitochondria play a