Titlebook: Nanoimaging; Methods and Protocol Alioscka A. Sousa,Michael J. Kruhlak Book 2013 Springer Science+Business Media, LLC 2013 electron miscros

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Super-Resolution Imaging by Localization Microscopy example, the system under investigation will be fixed HeLa cells transfected with the photo-switchable fluorescent protein PS-CFP2 targeted to the plasma membrane by fusion to the N-terminus of the protein kinase Lck.

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Cellular Nanoimaging by Cryo Electron Tomographyation. We define the instrumentation, materials, and protocols for cryo electron tomography of whole cells, including cell growth, specimen vitrification, microscope alignments, data acquisition, tomographic image reconstruction, and 3D visualization techniques.

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Large-Volume Reconstruction of Brain Tissue from High-Resolution Serial Section Images Acquired by Sμm × 64 μm × 10 μm, or larger, at 2 nm pixel size), including how to obtain large-area serial sections of uniform thickness from well-preserved brain tissue that is rapidly perfusion-fixed with mixed aldehydes, processed with a microwave-enhanced method, and embedded into epoxy resin.