Titlebook: NanoBiotechnology Protocols; Sandra J. Rosenthal,David W. Wright Book 2005 Humana Press 2005 DNA.biotechnology.cellular processes.drug dis

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Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells,ar-scale phenomena within intact, live cells. VACNFs are fabricated via a combination of microfabrication techniques and catalytic plasma-enhanced chemical vapor deposition. In this chapter, we discuss the synthesis of VACNFs and detail the methods for introducing these arrays into the intracellular

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1064-3745 es currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author‘s emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides

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Book 2005nthesis, dynamic cellular imaging, and biological assays. The author‘s emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and or

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Book 2005ations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.

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Quantum Dot-Encoded Beads,racies as high as 99.99% under favorable conditions. DNA hybridization studies demonstrate that the coding and target signals can be simultaneously read at the single-bead level. This spectral coding technology is expected to open new opportunities in gene expression studies, high-throughput screening, and medical diagnostics.

DEFT

Whole-Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies,d sit), and uses common laboratory equipment for detection (spectrophotometer). Preparation of the antibodynanoshell conjugates is described, along with the design and optimization of the whole-blood nanoshell-based immunoassay system.

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Surface-Functionalized Nanoparticles for Controlled Drug Delivery,e dialysis method—are used for nanoparticle formation of the resulting copolymer. Prepared nanoparticles having a size <200 nm and a hydrophilic surface layer can be applied for bone-specific drug delivery.

seruting

,Use of Nanobarcodes® Particles in Bioassays,y virtue of the difference in reflectivity of adjacent metal stripes. This chapter describes protocols for the attachment of biological molecules, and the subsequent use of the Nanobarcodes particles in bioassays.

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