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Titlebook: NMDA Receptors; Methods and Protocol Nail Burnashev,Pierre Szepetowski Book 2024Latest edition The Editor(s) (if applicable) and The Author

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Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/660155.jpg
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Transfection in Primary Cultured Neuronal Cells,Transfection allows the introduction of foreign nucleic acid into eukaryotic cells. It is an important tool in understanding the roles of NMDARs in neurons. Here we describe using lipofection-mediated transfection to introduce cDNA encoding NMDAR subunits into postmitotic rodent primary cortical neurons maintained in culture.
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978-1-0716-3832-3The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Analysis of Functional NMDA Receptors in Astrocytes,hodology on how to study the physiology of NMDA receptors selectively on astrocytes will be described in detail. Astrocytes are the main class of neuroglia that control transmitter and ion homeostasis, which link cerebral blood flow and neuronal energy demands, but also affect synaptic transmission directly.
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