Titlebook: NMDA Receptor Protocols; Min Li Book 1999 Humana Press 1999

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INCH

Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells,ional channels can be produced with only the NR1 subunit. These single-subunit channels, exhibit many of the key properties found in native channels including the direct permeability of calcium, voltage-dependent Mg. blockade of the ion channel, and binding sites for modulators, such as Zn., glycine

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Immunocytochemistry of NMDA Receptors, immunoperoxidase for light microscopy (LM), the same for electron microscopy (EM), and postembedding immunogold (.–.). General versions of these techniques, as well as related techniques (immunofluorescence, immunocytochemistry of cultures) have been discussed and compared for immunolabeling of all

冷漠

The Use of Ligand Binding in Assays of NMDA Receptor Function, receptor measured NMDA-sensitive [.H]glutamate binding (.. for review). However, [.H]glutamate has relatively low affinity for the receptor, and is also a substrate for transport processes in brain membranes, which can make it difficult to isolate receptor binding from ligand sequestration. The rec

CAGE

Calmodulin Modification of NMDA Receptors,ceptor ion channels (.). We demonstrated that calmodulin specifically binds to two distinct sites (CBS1 and CBS2) localized in the C-terminus of the NR1 subunits, causing a fourfold decrease in the channel open probability (.). The CBS1 (.. for calmodulin: 80 n.) covers most of the C-terminal region

maudtin

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Redox Sensitivity of NMDA Receptors,voked responses. In contrast, after oxidation, NMDA-evoked responses are decreased in size. In recent years, as endogenous sources of oxidizing and reducing agents have been discovered, redox modulation of protein function has been recognized to be an important physiologic, as well as pathologic mec

accrete

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NMDA Receptor Pharmacology and Analysis of Patch-Clamp Recordings,ed from whole-cell recordings and (.) analysis of single-channel recordings. Whole-cell currents are the average response of a large population of channels, whereas single-channel recordings document the gating behavior of a single protein. Analysis of single-channel recordings details the effects o